California Institute for Biodiversity Invertebrate and Fungal eDNA bio inventory and models

We take thousands of environmental DNA samples and metabarcode CO1 for invertebrates, ITS1 and ITS2 for fungi, and 16S for bacteria. 2000 of these samples are from CALeDNA surface soil, sediment, and water collections built up by community scientists, students, professors, and curators over the past 8 years. 1000 of these samples are from soil collections facilitated by CIB, collected by Adventure Scientists, Stillwater Science, Museum-led field teams, and many more collaborators.

We share the eDNA sequences and results of what we identified on the eDNA Explorer. The results from CIB to date are on live to check! The eDNA data from CALeDNA are distributed over several projects on eDNA Explorer - explore around!

We put all the results together and make random forest maps combining eDNA-based taxa with hundreds of Earth observations to address the following knowledge gaps for the entire state of California for the purpose of guiding future CIB expeditions:

• How does species richness vary across California?

  • Guide people to areas with lots of species to collect.

• How do biodiversity communities vary across California?

  • Select places to go in your potential sampling area that have the most different communities.

• Where are there DNA-leads of species that still need California collections made of them?

  • Maximize new collections by prioritizing areas with under-collected biodiversity.

Here are the maps and reports!

COMMUNITY RICHNESS AND SIMILARITY STUDIES:

Urban biodiversity and community similarity across California https://docs.google.com/document/d/1XAmAHkvpcJMmqrRJbNuYFxKn58DTsCowsDuRuNX7IME/edit?usp=sharing

1. Lecanoromycetes beta diversity: https://docs.google.com/document/d/12Xy1GuvE2NsVu_hBn9sWni5qc1M6lqpFXPxjJMF9Ey0/edit?usp=sharing

2. Suillaceae beta diversity: https://docs.google.com/document/d/1IM14hNZreIHArJ8Pa-STJczd6XvedKHq38Ex-9UwVOA/edit?usp=sharing

3. Boletales beta diversity: https://docs.google.com/document/d/1hDnhLrPTo-JOyrvDFNURxkFQn5mlspvT3gRK1jkEg5E/edit?usp=sharing

4. Pyronema beta diversity: https://docs.google.com/document/d/1usGyER6vvuHu8Z97bfoEky90RHjs6TH2TtpMu3dNBQM/edit?usp=sharing

5. Wilcoxina beta diversity: https://docs.google.com/document/d/14N4dqXNT4xb_db6RifIAGYwqV8FZ7qxznX4WyXUYduM/edit?usp=sharing

   CALIFORNIA’S COLLECTION GAPS

6. Using environmental DNA to identify and address gaps in California’s collections of biodiversity: https://docs.google.com/document/d/1W9ff3UvFOnGqFS7nYyG4yybIW6mA5IFHPXTiZT2Sloc/edit?tab=t.0